3HHC

Interferon-lambda is functionally an interferon but structurally related to the IL-10 family

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X12
Synchrotron site	EMBL/DESY, Hamburg
Beamline	X12
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.907
Spacegroup name	P 31 2 1
Unit cell lengths	91.118, 91.118, 150.209
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.863 - 2.800
R-factor	0.265
Rwork	0.261
R-free	0.31400
Structure solution method	MIR
RMSD bond length	0.003
RMSD bond angle	0.662
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.000	25.000	2.690
High resolution limit [Å]	2.800	5.580	2.600
Rmerge	0.062	0.045	0.308
Number of reflections	39210
<I/σ(I)>	24.389
Completeness [%]	91.3	99.4	48.4
Redundancy	5.8	6.3	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	285	28 % w/v PEG 4000, 200 mM ammonium sulfate, 3 % v/v isopropyl alcohol , pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 285K
1	VAPOR DIFFUSION, SITTING DROP	5.5	285	28 % w/v PEG 4000, 200 mM ammonium sulfate, 3 % v/v isopropyl alcohol , pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 285K
1	VAPOR DIFFUSION, SITTING DROP	5.5	285	28 % w/v PEG 4000, 200 mM ammonium sulfate, 3 % v/v isopropyl alcohol , pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 285K