3GXX
Structure of the SH2 domain of the Candida glabrata transcription elongation factor Spt6, crystal form B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-08-15 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97971 |
Spacegroup name | P 32 |
Unit cell lengths | 71.600, 71.600, 87.600 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 18.700 - 2.400 |
Rwork | 0.253 |
R-free | 0.28500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gxw |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 18513 | |
<I/σ(I)> | 45.1 | 7.7 |
Completeness [%] | 99.5 | 95.4 |
Redundancy | 3.9 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 100 mM bicine pH 8.0, 4,3 M NaCl and 5 mM Tris(2-Carboxyethyl) phosphine Hydro-chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K |