3GP8
Crystal structure of the binary complex of RecD2 with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-06-20 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.140, 90.320, 142.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.180 - 2.500 |
| R-factor | 0.23023 |
| Rwork | 0.228 |
| R-free | 0.26827 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3e1s |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.146 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.032 | 0.175 |
| Number of reflections | 24589 | |
| <I/σ(I)> | 33.7 | 8 |
| Completeness [%] | 98.8 | 91.8 |
| Redundancy | 4.9 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 15% PEG 8000, 2-6% Ethylene glycol, 100mM Tris-HCl pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 8000 | ||
| 2 | 1 | 1 | Ethylene glycol | ||
| 3 | 1 | 1 | Tris-HCl | ||
| 4 | 1 | 2 | PEG 8000 | ||
| 5 | 1 | 2 | Ethylene glycol | ||
| 6 | 1 | 2 | Tris-HCl |
