3GLP
1.23 A resolution X-ray structure of (GCUGCUGC)2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-12-14 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.808 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 36.960, 38.850, 77.720 |
Unit cell angles | 90.00, 89.98, 90.00 |
Refinement procedure
Resolution | 17.980 - 1.230 |
R-factor | 0.148 |
Rwork | 0.147 |
R-free | 0.18400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | A-RNA model |
RMSD bond length | 0.018 |
RMSD bond angle | 2.749 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.250 |
High resolution limit [Å] | 1.230 | 1.230 |
Number of reflections | 32055 | |
<I/σ(I)> | 21.3 | 5.7 |
Completeness [%] | 99.1 | 98.1 |
Redundancy | 7.1 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 292 | MgCl2, Li2SO4, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Magnesium chloride | ||
2 | 1 | 1 | Lithium sulfate | ||
3 | 1 | 2 | Magnesium chloride | ||
4 | 1 | 2 | Lithium sulfate |