3GI1
Crystal Structure of the laminin-binding protein Lbp of Streptococcus pyogenes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-08-20 |
Detector | MAR345DTB |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.624, 92.163, 70.614 |
Unit cell angles | 90.00, 106.27, 90.00 |
Refinement procedure
Resolution | 27.982 - 2.450 |
R-factor | 0.19 |
Rwork | 0.186 |
R-free | 0.25100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1toa 1k0f 1pq4 1xvl 2o1e 3hh8 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.560 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.33) |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 27.984 | 29.090 | 2.530 |
High resolution limit [Å] | 2.400 | 7.590 | 2.400 |
Rmerge | 0.098 | 0.042 | 0.597 |
Total number of observations | 4771 | 21627 | |
Number of reflections | 20587 | ||
<I/σ(I)> | 6.861 | 13.9 | 1.2 |
Completeness [%] | 99.9 | 97.9 | 100 |
Redundancy | 7.3 | 7.1 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 291 | 0.7ul of 37 mg/ml Lbp in PBS, pH7.4 + 0.002% Na-azide + 0.7ul of 30% PEG1500, VAPOR DIFFUSION, SITTING DROP, temperature 291K |