3GEC
Crystal structure of a tandem PAS domain fragment of Drosophila PERIOD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 114.950, 114.950, 85.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.680 - 4.000 |
| R-factor | 0.247 |
| Rwork | 0.244 |
| R-free | 0.31200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wa9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.600 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.680 | 4.100 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.055 | 0.422 |
| Number of reflections | 5578 | |
| <I/σ(I)> | 18.07 | 3.82 |
| Completeness [%] | 96.7 | 97.8 |
| Redundancy | 5.4 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.7 | 282 | 70mM Sodiumtartrate, 20mM Hepes, 5mM DTE, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 282K |
