3G7V
Islet Amyloid Polypeptide (IAPP or Amylin) fused to Maltose Binding Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 104.025, 96.407, 103.996 |
Unit cell angles | 90.00, 90.53, 90.00 |
Refinement procedure
Resolution | 73.920 - 1.860 |
R-factor | 0.178 |
Rwork | 0.176 |
R-free | 0.20500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mpd |
RMSD bond length | 0.012 |
RMSD bond angle | 1.313 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 90.000 | 90.000 | 1.920 |
High resolution limit [Å] | 1.850 | 3.990 | 1.850 |
Rmerge | 0.054 | 0.035 | 0.460 |
Number of reflections | 169517 | ||
<I/σ(I)> | 27.432 | ||
Completeness [%] | 98.4 | 99.6 | 96.9 |
Redundancy | 5.5 | 5.6 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 2.0 M Ammonium Sulfate, 0.1M Sodium Acetate pH 4.6, vapor diffusion, hanging drop, temperature 298K |