3G6D
Crystal structure of the complex between CNTO607 Fab and IL-13
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 115 |
Detector technology | CCD |
Collection date | 2006-12-22 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.540, 68.030, 182.350 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 3.200 |
R-factor | 0.213 |
Rwork | 0.213 |
R-free | 0.26342 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries 3G6A 1ijz |
RMSD bond length | 0.010 |
RMSD bond angle | 1.361 |
Data reduction software | d*TREK (9.6L) |
Data scaling software | d*TREK (9.6L) |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.000 | 3.310 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.161 | 0.220 |
Number of reflections | 13028 | |
<I/σ(I)> | 6.1 | 4.6 |
Completeness [%] | 91.5 | 88.2 |
Redundancy | 6 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.5 | 293 | 0.1 M Sodium acetate pH 4.5, 4.9 M Sodium formate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |