3G3F
Crystal structure of the GluR6 ligand binding domain dimer with glutamate and NaCl at 1.38 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-16 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.185, 114.153, 52.364 |
| Unit cell angles | 90.00, 115.31, 90.00 |
Refinement procedure
| Resolution | 28.705 - 1.377 |
| R-factor | 0.1533 |
| Rwork | 0.152 |
| R-free | 0.17490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s7y |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.179 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.430 |
| High resolution limit [Å] | 1.377 | 1.380 |
| Rmerge | 0.043 | 0.315 |
| Number of reflections | 194151 | |
| <I/σ(I)> | 14.4 | 2.41 |
| Completeness [%] | 94.2 | 84 |
| Redundancy | 1.5 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 16% PEG4K, 12% isopropanol, 0.1M NaCitrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
