3FYQ
Structure of Drosophila melanogaster talin IBS2 domain (residues 1981-2168)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2008-12-03 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9808, 0.9811, 0.9694 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 90.919, 40.079, 57.547 |
Unit cell angles | 90.00, 112.87, 90.00 |
Refinement procedure
Resolution | 36.150 - 1.950 |
R-factor | 0.20875 |
Rwork | 0.206 |
R-free | 0.25548 |
Structure solution method | MAD |
RMSD bond length | 0.022 |
RMSD bond angle | 1.740 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 13427 | |
Completeness [%] | 92.2 | 54 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.95 | 294 | 0.1M Bicine pH 9.95, 1-2M magnesium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 294K |