3FG9
The crystal structure of an universal stress protein UspA family protein from Lactobacillus plantarum WCFS1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97942 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.355, 118.949, 191.974 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.600 - 1.470 |
R-factor | 0.17907 |
Rwork | 0.177 |
R-free | 0.21235 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.426 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXD |
Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.600 | 1.500 |
High resolution limit [Å] | 1.470 | 1.470 |
Rmerge | 0.068 | 0.558 |
Number of reflections | 149737 | |
<I/σ(I)> | 46.3 | 2.6 |
Completeness [%] | 99.5 | 92.7 |
Redundancy | 8.4 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2M MgActate. 20% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 289K |