3FG6
Structure of the C-terminus of Adseverin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 2007-03-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 67.644, 90.384, 98.839 |
Unit cell angles | 88.79, 88.26, 76.26 |
Refinement procedure
Resolution | 29.920 - 3.000 |
R-factor | 0.247 |
Rwork | 0.244 |
R-free | 0.29500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1p8x |
RMSD bond length | 0.007 |
RMSD bond angle | 1.002 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.040 | 0.142 |
Number of reflections | 41218 | |
<I/σ(I)> | 16.784 | |
Completeness [%] | 90.3 | 85 |
Redundancy | 1.9 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 20% PEG 4000, 200mM sodium isothiocyanate, VAPOR DIFFUSION, SITTING DROP, temperature 289K |