3FEL
Crystal structure of the R132K:R111L:T54E mutant of cellular retinoic acid-binding protein II at 1.85 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-13 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97850 |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.803, 36.678, 56.892 |
| Unit cell angles | 75.52, 74.88, 88.40 |
Refinement procedure
| Resolution | 35.490 - 1.850 |
| R-factor | 0.19278 |
| Rwork | 0.190 |
| R-free | 0.24847 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g7b |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.238 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.200 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.051 | 0.297 |
| Number of reflections | 18943 | |
| <I/σ(I)> | 26.6 | 2.4 |
| Completeness [%] | 84.5 | 43.3 |
| Redundancy | 2.4 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M BTP, 30% PEG4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
