3F9V
Crystal Structure Of A Near Full-Length Archaeal MCM: Functional Insights For An AAA+ Hexameric Helicase
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2007-10-29 |
Detector | ADSC |
Wavelength(s) | 0.97940 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 202.526, 202.526, 128.243 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 4.350 |
R-factor | 0.415 |
Rwork | 0.412 |
R-free | 0.48100 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.195 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 4.350 |
Number of reflections | 7760 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 50mM NaCl, 65mM CalCl2, 20% MPD, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |