3F51
Crystal Structure of the clp gene regulator ClgR from Corynebacterium glutamicum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2007-11-09 |
Detector | PHILLIPS |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.440, 84.820, 71.430 |
Unit cell angles | 90.00, 95.87, 90.00 |
Refinement procedure
Resolution | 45.740 - 2.050 |
R-factor | 0.21359 |
Rwork | 0.212 |
R-free | 0.23801 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.465 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.000 | 2.150 |
High resolution limit [Å] | 2.050 | 2.050 |
Number of reflections | 48625 | |
<I/σ(I)> | 16 | 2.8 |
Completeness [%] | 99.7 | 99.8 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 0.1 M sodium chloride, 23% 2-methyl-2,4-pentanediol, 15% glycerol, 0.085 M sodium acetate , pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 0.1 M sodium chloride, 23% 2-methyl-2,4-pentanediol, 15% glycerol, 0.085 M sodium acetate , pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |