3EYA
Structural basis for membrane binding and catalytic activation of the peripheral membrane enzyme pyruvate oxidase from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-22 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.91840 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 203.243, 207.051, 214.542 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.500 |
R-factor | 0.184 |
Rwork | 0.183 |
R-free | 0.19800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ey9 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.467 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.21) |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 84.820 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.132 | 0.704 |
Total number of observations | 204910 | |
Number of reflections | 309368 | |
<I/σ(I)> | 4.722 | 1.1 |
Completeness [%] | 99.6 | 98.4 |
Redundancy | 5.2 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 286 | 0.1M MES/NaOH, 20-35% 2-methyl-2,4-pentanediol (MPD), pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 286K |