3EWL
Crystal Structure of Conserved protein BF1870 of Unknown Function from Bacteroides fragilis
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-13 |
| Detector | SBC-3 |
| Wavelength(s) | 0.9793, 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.905, 41.606, 65.600 |
| Unit cell angles | 90.00, 105.51, 90.00 |
Refinement procedure
| Resolution | 29.660 - 2.000 |
| R-factor | 0.187 |
| Rwork | 0.184 |
| R-free | 0.24100 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.596 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.4.0078) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.660 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.101 | 0.329 |
| Number of reflections | 15373 | |
| <I/σ(I)> | 7.5 | 3 |
| Completeness [%] | 98.9 | 88.9 |
| Redundancy | 4.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.2M Trimethylamine N-oxide dihydrate, 0.1M Tris pH 8.5, 20% w/v Polyethylene glycol monomethyl ether 2000, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
