3EUR
Crystal structure of the C-terminal domain of uncharacterized protein from Bacteroides fragilis NCTC 9343
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-09-30 |
Detector | SBC-3 |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.145, 43.428, 98.692 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.330 - 1.300 |
R-factor | 0.14032 |
Rwork | 0.139 |
R-free | 0.17009 |
Structure solution method | SAD |
RMSD bond length | 0.019 |
RMSD bond angle | 1.711 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.334 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.043 | 0.150 |
Number of reflections | 32763 | |
<I/σ(I)> | 44.7 | 5.14 |
Completeness [%] | 91.2 | 50 |
Redundancy | 8 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.2 M Potassium sodium tartrate tetrahydrate 20% Peg 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |