3ET6
The crystal structure of the catalytic domain of a eukaryotic guanylate cyclase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 123.678, 123.678, 62.822 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.000 - 2.550 |
R-factor | 0.1738 |
Rwork | 0.172 |
R-free | 0.21500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1azs |
RMSD bond length | 0.015 |
RMSD bond angle | 23.839 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.25) |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 28.006 | 28.010 | 2.690 |
High resolution limit [Å] | 2.550 | 8.060 | 2.550 |
Rmerge | 0.074 | 0.027 | 0.521 |
Number of reflections | 17490 | ||
<I/σ(I)> | 7.612 | 22.2 | 1.3 |
Completeness [%] | 96.2 | 90.9 | 98.2 |
Redundancy | 4.3 | 4.4 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 0.1 M sodium cacodylate, pH 5.0 - 6.4, 42-62% saturated (NH4)2HPO4, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |