3EIW

Crystal structure of Staphylococcus aureus lipoprotein, HtsA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-12-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97934
Spacegroup name	P 1 21 1
Unit cell lengths	44.703, 43.571, 75.712
Unit cell angles	90.00, 100.59, 90.00

Refinement procedure

Resolution	50.000 - 1.600
R-factor	0.16511
Rwork	0.163
R-free	0.20001
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.374
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (refmac_5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.660
High resolution limit [Å]	1.600	3.450	1.600
Rmerge	0.059	0.042	0.166
Number of reflections	36952
<I/σ(I)>	20.809
Completeness [%]	96.8	99.6	76.5
Redundancy	3.4	3.4	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	0.1 Hepes pH 7.0, Jeffamine ED-2001, VAPOR DIFFUSION, HANGING DROP, temperature 295K