3EHN
BT1043 with N-acetyllactosamine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 150 |
Detector technology | CCD |
Collection date | 2008-03-20 |
Detector | SBC-3 |
Wavelength(s) | 0.97167 |
Spacegroup name | P 21 3 |
Unit cell lengths | 156.090, 156.090, 156.090 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.800 |
Rwork | 0.196 |
R-free | 0.24600 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.329 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.750 | 2.750 |
Number of reflections | 32650 | |
<I/σ(I)> | 20.5 | 3.4 |
Completeness [%] | 98.4 | 90.6 |
Redundancy | 8.3 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 12-14% polyethylene glycol 4000, 50mM Na/K phosphate, 100mM succinate, 120mM N-acetyllactosamine, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |