3ECA
CRYSTAL STRUCTURE OF ESCHERICHIA COLI L-ASPARAGINASE, AN ENZYME USED IN CANCER THERAPY (ELSPAR)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR590 |
Temperature [K] | 293 |
Detector technology | AREA DETECTOR |
Collection date | 1992-01-01 |
Detector | SIEMENS-XENTRONICS |
Wavelength(s) | 1.542 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 76.700, 96.100, 111.300 |
Unit cell angles | 90.00, 97.10, 90.00 |
Refinement procedure
Resolution | 9.990 - 2.400 |
R-factor | 0.149 * |
Rwork | 0.111 |
Structure solution method | MIR |
RMSD bond length | 0.016 |
RMSD bond angle | 0.054 * |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | X-PLOR |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | |
High resolution limit [Å] | 2.300 * | |
Rmerge | 0.055 | |
Completeness [%] | 74.0 * | |
Redundancy | 5.4 | 5.4 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.1 M sodium acetate, MPD, PEG3350 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | sodium acetate | 0.1 (M) | |
2 | 1 | reservoir | sodium acetate | 0.1 (M) | |
3 | 1 | reservoir | MPD | ||
4 | 1 | reservoir | PEG3350 | 25 (%) |