3E66
Crystal structure of the beta-finger domain of yeast Prp8
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-09-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.169, 84.810, 94.008 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.050 |
R-factor | 0.214 |
Rwork | 0.214 |
R-free | 0.23600 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.239 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.120 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.101 | 0.322 |
Number of reflections | 37829 | |
<I/σ(I)> | 33.8 | 2.8 |
Completeness [%] | 94.5 | 68.8 |
Redundancy | 12.4 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M Tris-HCl pH 8.5, 10% PEG 8000, 0.2M Li2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |