3E42
Q138F HincII bound to GTCGAC and Ca2+ (cocrystallized)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Collection date | 2007-07-01 |
Wavelength(s) | 0.94219 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.030, 90.140, 66.440 |
Unit cell angles | 90.00, 104.40, 90.00 |
Refinement procedure
Resolution | 48.458 - 2.680 |
R-factor | 0.192 |
Rwork | 0.188 |
R-free | 0.28300 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.217 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.750 |
High resolution limit [Å] | 2.680 | 2.680 |
Rmerge | 0.048 | 0.373 |
Number of reflections | 15869 | |
<I/σ(I)> | 12.4 | 2 |
Completeness [%] | 98.5 | 97.9 |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 20% PEG 4000, 150 mM NaCl, 5 mM CaCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 4000 | ||
2 | 1 | 1 | NaCl | ||
3 | 1 | 1 | CaCl2 | ||
4 | 1 | 2 | PEG 4000 | ||
5 | 1 | 2 | NaCl | ||
6 | 1 | 2 | CaCl2 |