3E0E
Crystal structure of a domain of replication protein A from Methanococcus maripaludis. NorthEast Structural Genomics targe MrR110B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-29 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 25.263, 54.896, 56.223 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.280 - 1.600 |
R-factor | 0.234 |
Rwork | 0.234 |
R-free | 0.26900 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXS |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.048 | 0.225 |
Number of reflections | 19963 | |
<I/σ(I)> | 19.5 | |
Completeness [%] | 99.7 | 98.7 |
Redundancy | 21.4 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5 | 293 | Sodium chloride 0.1 M, Sodium Acetate 0.1 M, PEG 4000 40%, pH 5, Micro batch under oil method, temperature 293K | |
1 | 5 | 293 | Sodium chloride 0.1 M, Sodium Acetate 0.1 M, PEG 4000 40%, pH 5, Micro batch under oil method, temperature 293K |