3DXM
Structure of Bos taurus Arp2/3 Complex with Bound Inhibitor CK0993548
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-07-25 |
Detector | MARRESEARCH |
Wavelength(s) | 1.08 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 111.383, 129.651, 203.787 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.850 |
R-factor | 0.246 |
Rwork | 0.245 |
R-free | 0.25800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k8k |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.950 |
High resolution limit [Å] | 2.850 | 2.850 |
Number of reflections | 69642 | |
<I/σ(I)> | 2.2 | |
Completeness [%] | 96.8 | 91.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 8% PEG 8000, 50 mM HEPES, pH 7.5,100 mM KSCN, VAPOR DIFFUSION, HANGING DROP, temperature 277K |