3DPT
COR domain of Rab family protein (Roco)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97955 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 130.112, 130.112, 129.199 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.830 - 2.900 |
| R-factor | 0.21136 |
| Rwork | 0.210 |
| R-free | 0.24282 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.127 | 0.699 |
| Number of reflections | 28455 | |
| <I/σ(I)> | 12.7 | 5.23 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.8 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 600mM Succinate (pH6.5), 5% 2-Methyl-2,4-pentan-diol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
