3DMW
Crystal structure of human type III collagen G982-G1023 containing C-terminal cystine knot
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-12-12 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0000, 0.979, 1.02, 0.97963 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.980, 21.520, 68.970 |
Unit cell angles | 90.00, 92.58, 90.00 |
Refinement procedure
Resolution | 34.450 - 2.300 |
R-factor | 0.248 |
Rwork | 0.248 |
R-free | 0.27400 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.900 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.450 | 2.440 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.075 | 0.240 |
Number of reflections | 3964 | |
<I/σ(I)> | 7.3 | 2.3 |
Completeness [%] | 90.0 | 89 |
Redundancy | 5 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 30% PEG 550, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |