3DIV
Crystal structure of laccase from Cerrena maxima at 1.76A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-12 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.8068 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.581, 77.101, 130.884 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 7.990 - 1.760 |
R-factor | 0.18067 |
Rwork | 0.176 |
R-free | 0.21705 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2h5u |
RMSD bond length | 0.011 |
RMSD bond angle | 1.399 |
Data reduction software | DENZO |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.070 | 1.800 |
High resolution limit [Å] | 1.760 | 1.760 |
Rmerge | 0.180 | |
Number of reflections | 52442 | |
Completeness [%] | 96.0 | 91.8 |
Redundancy | 4.87 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 50mM sodium citrate, 15%(w/v) PEG 6000, 0.04M Tris, malein, NaOH , pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |