3D1E
Crystal structure of E. coli sliding clamp (beta) bound to a polymerase II peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.959 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.139, 69.869, 73.119 |
| Unit cell angles | 112.89, 91.08, 99.28 |
Refinement procedure
| Resolution | 33.550 - 1.900 |
| Rwork | 0.226 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pol |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.253 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.780 |
| Rmerge | 0.058 |
| Number of reflections | 60707 |
| <I/σ(I)> | 11.3 |
| Completeness [%] | 94.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.2 | 295 | 27.5% PEG400, 100 mM MES pH 6.2, 100 mM calcium chloride and 1% DMSO, VAPOR DIFFUSION, temperature 295K |
