3CYM
Crystal structure of protein BAD_0989 from Bifidobacterium adolescentis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2008-03-21 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.163, 94.422, 113.196 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.100 |
R-factor | 0.22417 |
Rwork | 0.222 |
R-free | 0.29405 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.111 | 0.860 |
Number of reflections | 34422 | |
<I/σ(I)> | 5.1 | 1.2 |
Completeness [%] | 94.1 | 96.8 |
Redundancy | 5.7 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 100mM HEPES pH 7.5, 10% PEG 3350, 200mM Proline, 10% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 294K |