3CWZ
Structure of RAB6(GTP)-R6IP1 complex
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.978 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 100.240, 100.240, 299.250 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.970 - 3.200 |
R-factor | 0.246 |
Rwork | 0.246 |
R-free | 0.30200 |
Structure solution method | SAD |
RMSD bond length | 2.823 |
RMSD bond angle | 1.700 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.000 |
Rmerge | 0.060 |
Number of reflections | 18629 |
<I/σ(I)> | 13.3 |
Completeness [%] | 99.6 |
Redundancy | 9.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.1 M HEPES, 2% PEG 4000, 3% MPD, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.1 M HEPES, 2% PEG 4000, 3% MPD, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 291K |