3CSV

Crystal structure of a putative aminoglycoside phosphotransferase (YP_614837.1) from Silicibacter sp. TM1040 at 2.15 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-03-17
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97915
Spacegroup name	C 1 2 1
Unit cell lengths	113.680, 55.940, 61.220
Unit cell angles	90.00, 102.90, 90.00

Refinement procedure

Resolution	29.735 - 2.150
R-factor	0.171
Rwork	0.168
R-free	0.22800
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.174
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.735	29.735	2.230
High resolution limit [Å]	2.150	4.610	2.150
Rmerge	0.055	0.026	0.322
Number of reflections	20496	3938	4013
<I/σ(I)>	10.33	24.1	2.4
Completeness [%]	98.2	97.4	96.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	NANODROP, 2.0M (NH4)2SO4, 0.2M NaCl, 0.1M Cacodylate pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K