3CSP

Crystal structure of the DM2 mutant of myelin oligodendrocyte glycoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-28
Detector	MAR CCD 165 mm
Wavelength(s)	0.91841
Spacegroup name	P 32 2 1
Unit cell lengths	49.240, 49.240, 77.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	17.900 - 1.700
R-factor	0.201
Rwork	0.199
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.660
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.900	1.800
High resolution limit [Å]	1.700	1.700
Rmerge	0.049	0.311
Number of reflections	12373
Completeness [%]	99.3	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	295	100 mM Tris/HCl, 12% PEG 8000, 100 mM magnesium acetate, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 295K