3CPA
X-RAY CRYSTALLOGRAPHIC INVESTIGATION OF SUBSTRATE BINDING TO CARBOXYPEPTIDASE A AT SUBZERO TEMPERATURE
Replaces: 1CPAExperimental procedure
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.600, 60.270, 47.250 |
| Unit cell angles | 90.00, 97.27, 90.00 |
Refinement procedure
| Resolution | ? - 2.000 |
| R-factor | 0.162 * |
| Refinement software | PROLSQ |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 7.4 * | 4 * | THE COMPLEX WAS PREPARED BY DIFFUSION OF GLY-TYR INTO CPA CRYSTALS. THE OCCUPANCY OF THE GLY-TYR IS APPROXIMATELY FORTY PERCENT. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | 1.2 (M) | ||
| 2 | 1 | 1 | Tris-HCl | 0.02 (M) | |
| 3 | 1 | 2 | 0.2 (M) | ||
| 4 | 1 | 2 | Tris-HCl | 0.02 (M) |
