3CMO
HIV neutralizing monoclonal antibody YZ18
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-07 |
Detector | CUSTOM-MADE |
Wavelength(s) | 1.24 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 75.650, 69.940, 84.360 |
Unit cell angles | 90.00, 94.93, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.300 |
Rwork | 0.248 |
R-free | 0.28700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yeh |
RMSD bond angle | 0.007 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 37445 | |
<I/σ(I)> | 5.9 | 1.9 |
Completeness [%] | 95.5 | 95 |
Redundancy | 3 | 2.95 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 18% PEG6000, 0.1 M sodium citrate, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |