3CKF
The crystal structure of OspA deletion mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-01-01 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97934 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 34.303, 53.910, 55.854 |
Unit cell angles | 90.00, 95.76, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.250 |
R-factor | 0.1578 |
Rwork | 0.156 |
R-free | 0.19170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g8c |
RMSD bond length | 0.014 |
RMSD bond angle | 1.465 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.290 |
High resolution limit [Å] | 1.250 | 1.250 |
Rmerge | 0.066 | 0.231 |
Number of reflections | 54134 | |
<I/σ(I)> | 22 | 5.18 |
Completeness [%] | 96.4 | 84.5 |
Redundancy | 3.9 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.45 | 293 | 38% PEG400, 0.1M Imidazole, 0.05M MgSO4, 11mg/ml, pH 6.45, VAPOR DIFFUSION, HANGING DROP, temperature 293K |