3CJR
Ribosomal protein L11 methyltransferase (PrmA) in complex with ribosomal protein L11 (K39A) and inhibitor Sinefungin.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9797 |
Spacegroup name | P 65 |
Unit cell lengths | 132.742, 132.742, 46.056 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.050 |
R-factor | 0.21636 |
Rwork | 0.213 |
R-free | 0.27695 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | pdb entries 2NXC 2nxn |
RMSD bond length | 0.006 |
RMSD bond angle | 1.125 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.120 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.067 | 0.528 |
Number of reflections | 27841 | |
<I/σ(I)> | 18.7 | 2.3 |
Completeness [%] | 99.7 | 99 |
Redundancy | 3.7 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | microbatch under oil | 8.5 | 277 | 170 mM sodium acetate trihydrate, 85 mM TRIS-HCl, 25.5 (w/v) PEG 4000, 15% (v/v) glycerol, 1 mM Sinefungin, pH 8.5, microbatch under oil, temperature 277K |