3CBQ

Crystal structure of the human REM2 GTPase with bound GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-02-07
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	64.143, 64.330, 73.204
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.710 - 1.820
R-factor	0.192
Rwork	0.190
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gjs
RMSD bond length	0.016
RMSD bond angle	1.437
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (refmac_5.3.0037)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.000	25.000	1.890
High resolution limit [Å]	1.820	3.920	1.820
Rmerge	0.074	0.033	0.658
Number of reflections	13866
<I/σ(I)>	10.6
Completeness [%]	99.8	99.9	99
Redundancy	6.9	6.8	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	20% PEG 3350, 0.2M KCl, VAPOR DIFFUSION, temperature 291K