3C7K
Molecular architecture of Galphao and the structural basis for RGS16-mediated deactivation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-10-01 |
Detector | BRANDEIS - B4 |
Wavelength(s) | 1.15 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 96.357, 96.357, 235.610 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.900 |
Rwork | 0.250 |
R-free | 0.31100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1agr |
RMSD bond length | 0.009 |
RMSD bond angle | 1.346 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.900 |
Number of reflections | 27489 |
<I/σ(I)> | 20.2 |
Completeness [%] | 83.0 |
Redundancy | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 15% PEG 8000 200 mM Tris pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |