3C4T

Structure of RNaseIIIb and dsRNA binding domains of mouse Dicer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-05
Detector	ADSC QUANTUM 210
Wavelength(s)	1.11
Spacegroup name	P 42 21 2
Unit cell lengths	70.126, 70.126, 132.663
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.800
R-factor	0.23568
Rwork	0.233
R-free	0.30429
RMSD bond length	0.019
RMSD bond angle	2.163
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.4.0067)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.850
High resolution limit [Å]	2.750	2.750
Rmerge	0.057	0.368
Number of reflections	7819
<I/σ(I)>	28.6	3.67
Completeness [%]	86.0	46
Redundancy	7.5	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	20-26% PEG400, 100 mM HEPES (pH7.0), VAPOR DIFFUSION, HANGING DROP, temperature 295K