3C1E
Crystal structure of Staphylococcal nuclease variant Delta+PHS L125K at cryogenic temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OTHER |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2008-01-10 |
Detector | BRUKER APEX II |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.075, 60.453, 38.144 |
Unit cell angles | 90.00, 93.10, 90.00 |
Refinement procedure
Resolution | 27.610 - 1.900 |
R-factor | 0.201 |
Rwork | 0.195 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1TQO.PDB |
RMSD bond length | 0.011 |
RMSD bond angle | 1.434 |
Data reduction software | SAINT |
Data scaling software | SAINT (V7.34A) |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.090 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.036 | 0.171 |
Number of reflections | 11202 | |
<I/σ(I)> | 38.45 | 6.05 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 11.09 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 32% MPD, 25 mM Potassium Phosphate, Calcium Chloride, pdTp, pH 9.0, vapor diffusion, hanging drop, temperature 277K |