3BZZ
Crystal structural of the mutated R313T EscU/SpaS C-terminal domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-01-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 31.297, 48.606, 62.464 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.350 - 1.407 |
R-factor | 0.182 |
Rwork | 0.180 |
R-free | 0.21900 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.414 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.460 |
High resolution limit [Å] | 1.407 | 3.040 | 1.407 |
Rmerge | 0.065 | 0.040 | 0.378 |
Number of reflections | 18717 | ||
<I/σ(I)> | 10.6 | ||
Completeness [%] | 97.6 | 96.3 | 83.3 |
Redundancy | 6.9 | 7 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6.5 | 293 | (NH4)2SO4, Bis-Tris, Pentaerythriol Ethoxylate, NaCl, pH 6.5, Microbatch, temperature 293K |