3BT3
Crystal structure of a glyoxalase-related enzyme from Clostridium phytofermentans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97960 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 86.410, 87.100, 50.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.820 - 2.500 |
R-factor | 0.222 |
Rwork | 0.222 |
R-free | 0.27000 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.300 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.820 | 1.780 |
High resolution limit [Å] | 1.690 | 1.690 |
Rmerge | 0.152 | 0.800 |
Number of reflections | 43640 | |
<I/σ(I)> | 6.5 | 2.6 |
Completeness [%] | 99.9 | 100 |
Redundancy | 13.4 | 13.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2M MgCl2, Bis-Tris Buffer, 25% PEG 3350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |