3BDR
Crystal structure of fatty acid-binding protein-like Ycf58 from Thermosynecoccus elongatus. Northeast Structural Genomics Consortium target TeR13.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-07-06 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97900 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 75.014, 75.014, 83.359 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.400 - 2.800 |
R-factor | 0.236 |
Rwork | 0.236 |
R-free | 0.26200 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.081 | 0.691 |
Number of reflections | 11252 | |
<I/σ(I)> | 28.2 | 4.6 |
Completeness [%] | 99.9 | 100 |
Redundancy | 10.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 10 | 293 | 18% PEG 20000, 0.1M NH4H2PO4, 0.1M CAPS, 20% Glycerol, pH 10.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |