3BC9
Alpha-amylase B in complex with acarbose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-27 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9795 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 227.850, 77.240, 50.380 |
Unit cell angles | 90.00, 98.63, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.350 |
R-factor | 0.1514 |
Rwork | 0.151 |
R-free | 0.17814 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.022 |
RMSD bond angle | 1.941 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.400 |
High resolution limit [Å] | 1.350 | 1.350 |
Number of reflections | 188142 | |
<I/σ(I)> | 10.9 | 2.2 |
Completeness [%] | 99.5 | 98.3 |
Redundancy | 4 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.2M sodium acetate, 0.1M Mes-OH, 20% (w/v) PEG 8000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |