3BC2
METALLO BETA-LACTAMASE II FROM BACILLUS CEREUS 569/H/9 AT PH 6.0, MONOCLINIC CRYSTAL FORM
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 153 |
| Detector technology | IMAGE PLATE AREA DETECTOR |
| Collection date | 1996-08 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 53.440, 61.600, 69.830 |
| Unit cell angles | 90.00, 93.27, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.700 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | FOR 1BC2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.394 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.300 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 30934 | |
| <I/σ(I)> | 5.14 | 2.2 |
| Completeness [%] | 96.4 | 94.5 |
| Redundancy | 2.4 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | pH 6.0 |
