3BBI
Minimally Junctioned Hairpin Ribozyme Incorporating A38(2AP) and A-1 2'-O-Me Modifications near Active Site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-06-26 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 93.250, 93.250, 132.830 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 23.080 - 2.350 |
R-factor | 0.2281 |
Rwork | 0.228 |
R-free | 0.23800 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2oue |
RMSD bond length | 0.007 |
RMSD bond angle | 1.500 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.310 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 14740 | |
<I/σ(I)> | 23.4 | 3.3 |
Completeness [%] | 99.6 | 100 |
Redundancy | 6.5 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | PEG 2K MME, lithium sulfate, HEPES, spermidine, cobalt hexaammine, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 2K MME | ||
2 | 1 | 1 | Li2SO4 | ||
3 | 1 | 1 | HEPES | ||
4 | 1 | 1 | spermidine | ||
5 | 1 | 1 | cobalt hexaammine | ||
6 | 1 | 2 | PEG 2K MME | ||
7 | 1 | 2 | Li2SO4 | ||
8 | 1 | 2 | HEPES | ||
9 | 1 | 2 | spermidine |