3B97

Crystal Structure of human Enolase 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-09-06
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 42
Unit cell lengths	192.796, 192.796, 65.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.200
R-factor	0.187
Rwork	0.184
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.460
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS (0.9)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200
Rmerge	0.087
Number of reflections	118254
Completeness [%]	97.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	100mM Tris-HCl, 20-24% (w/v) PEG 3350, 200mM ammonium sulfate, 1mM DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K