3B84
Crystal structure of the human BTB domain of the Krueppel related Zinc Finger Protein 3 (HKR3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-26 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.98248 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 50.375, 50.375, 86.093 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.640 - 1.740 |
| R-factor | 0.188 |
| Rwork | 0.186 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nn2 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.548 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.084 | 0.761 |
| Number of reflections | 13630 | |
| <I/σ(I)> | 10.6 | 2.075 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.3 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2M MgCl2, 0.1M Bis-tris pH 6.5, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
